- Description
- Specifications
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Description
Product Overview:
BIOGRO-2 formulation is totally free of serum albumin and the concentration of total protein is therefore less than 25 micrograms per ml in the final media.
Applications:
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- Culture of murine myeloma cells (NS-1, NS-0/1, SP2)
- Culture of mouse-mouse hybridoma cells (from NS-1, NS-0/1, SP2, X63-Aag8-653, BW-S147)
- Culture of rat-mouse, rat-rat, and human-human hybridomas
- Monoclonal antibody production
- Culture of other human, murine and avian lymphocytes
- Viral production (such as HEV virus from Turkey B-lymphoblastoid cell lines)
- Culture of various anchorage-dependent cells (such as human osteosarcoma, human epithelial, and thyroid cells)
- Maintenance of most anchorage-dependent cells during long production periods
Method of use:
- BIOGRO-2 is 50 fold concentrates and therefore is recommended for use at a concentration of 2% (although in some cases 1% may be sufficient). DMEM F-12 (1:1) has found to be most generally effective as the basal media, but many cell lines grow well with RPMI 1640, DMEM media or Iscove’s. Glutamine and antibiotics should be added to the final formulation.
Additional information
| Quantity | 100 mL |
|---|---|
| Glucose | No Glucose |
| Storage Conditions | -20ºC |
| Shipping Conditions | Dry Ice |
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