DCCM-1 SFM

Description

DCCM-1 SFM:

DCCM-1 is serum-free medium, specially optimised for the growth of hybridoma cells and for monoclonal antibody production. This medium is a high protein formulation designed to maximise the growth of cells, and it is ready-to-use and needs only the addition of L-Glutamine. DCCM-1 contains BSA.

Applications

• Culture of myeloma and hybridoma cells
• Monoclonal antibody production
• Culture of human lymphocyte cells (including stimulated or transformed cells)

Additional information

1. McClelland AR, Barrow D, Madden TA, et al., Enhanced Epidermal Growth Factor Receptor Signaling in MCF7 Breast Cancer Cell after Long-Term Culture in the presence of the Pure Antiestrogen ICI 182,780 (Faslodex). Endocrinology 142 (7): 2776-2788 (2001)
2. Suarez Y, Fernandez C, Ledo B, et al., Differential effects of ergosterol and cholesterol on Cdk1 activation and SRE-driven transcription. Eur. J. Biochem. 269 (6): 1761-1771 (2002)
3. Teitelbaum D, Arnon R, Sela M, et al., Immunomodulation of experimental autoimunne encephalomyelitis by oral administration of copolymer 1. Proc. Natl. Acad. Sci. USA 96 (7): 3842-3847 (1999).
4. Rotem-Yehudar R, Galperin E, Horowitz M. Association of Insulin- like Growth Factor 1 Receptor with EHD1 and SNAP29.  J. Biol. Chem. 276 (35): 33054-33060 (2001).
5. Fares FA, Levi F, Reznick AZ, Kraiem Z. Engineering a Potential Antagonist of Human Thyrotropin and Thyroid-stimulating Antibody. J. Biol. Chem. 276 (7): 4543-4548 (2001).
6. Kraiem Z, Sadeh O, Yosef M. Iodide uptake and origanification, tri-iodothyronine secretion, cyclic AMP accumulation and cell proliferation in an optimized system of human thyroid follicles cultured in collagen gel suspended in serum-free medium. Journal of Endocrinology 131(3): 499-506 (1991).
7. Shirvan A, Ziv I, Machlin T, et al., Two Waves of Cyclin B and Proliferating Cell Nuclear Antigen Expression During Dopamine-Triggered Neuronal Apoptosis. J. Neurochem. 69 (2): 539-549 (1997).
8. Heinrich R, Kraiem Z. The Protein Kinase A Pathway Inhibits c-jun and c-fos Protooncogene Expression Induced by the Protein Kinase C and Tyrosine Kinase Pathways in Cultured Human Thyroid Follicles. Journal of Clinical Endocrinology and Metabolism 82 (6): 1839-1844 (1997).
9. Leshem B, Brass D. Mouse lymphoblasts lose their immunogenicity and susceptibility to specific cytotoxic T lymphocyte lysis during maintenance in culture. Immunology 95(3): 409-418 (1998).
10. Levodopa Induces Apoptosis in Cultured Neutronal Cells – A Possible Accelerator of Nigrostriatal Degeneration in Parkinson’s Disease?.  Movement Disorders 12 (1): 17-23 (1997).
11. Lazarov-Spiegler O, Solomon AS, Zeev-Brann AB, et al., Transplantation of activated macrophages overcomes central nervous system regrowth failure. FASEB J. 10: 1296-1302  (1996).
12. Kraiem Z, Sadeh O, Yosef M, et al., Mutual Antagonistic Interactions between the Thyrotropin (Adenosine 3’, 5’- Monophosphate) and Protein Kinase C/Epidermal Growth Factor (Tyrosine Kinase) Pathways in Cell Proliferation and Differentiation of Cultured Human Thyroid Follicles. Endocrinology 136 (2): 585-590
13. Richards R J, Oreffo I C V, Lewis W R. Clara cell culture from the mouse and their reaction to bronchiola toxins. Environmental Health Perspectives 85: 119-127 (1990).

Materials Safety Data Sheet

• DCCM-1 SFM (MSDS)

Technical Guide

• DCCM-1 SFM (Technical Guide)