EZ-PCR Mycoplasma Test Kit

Description

EZ-PCR Mycoplasma Test Kit Overview:

The EZ-PCR Mycoplasma Test Kit is a highly-sensitive and specific PCR-based assay capable of detecting a broad range of mycoplasma species in cell cultures and other biological materials. This kit contains an optimized, complete PCR mix, including a variety of mycoplasma-specific primers, dNTP mix, and Taq polymerase. The simple protocol and ready-to-use system means that samples can be prepared and run in just 10 minutes, with comprehensive and accurate results available within a couple of hours.

Mycoplasma testing should be performed routinely every 3 to 6 months and when incorporating new cultures from external sources into your laboratory.

Mycoplasma

• Detects a variety of mycoplasma species: M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum, and Acholeplasma and Spiroplasma species, with high sensitivity and specificity.
• Does not interfere with media components.

Advantages

• Samples are easy to prepare
• Fast, reproducible, and reliable results
• No need for internal control application steps
• No loading dye needed for the agarose gel

Kit Components

Reaction mix: 200 µL
Buffer solution: 1 mL
Positive template control : 20 µL

Additional information

1. J. Rosati et al. Production and characterization of human induced pluripotent stem cells (iPSCs) from Joubert Syndrome: CSSi001-A (2850). Stem Cell Research, Volume 27, March 2018, Pages 74–77
2. Gazit, et al. Recognition of Mycoplasma hyorhinis by CD99-Fc molecule. European Journal of Immunology, 34 (7): 2032-2040 (2004)
3. Johansson, et al. Platelet Lysate: a Replacement for Fetal Bovine Serum in Animal Cell Culture, Cytotechnology 42 (2): 67-74 (2003)
4. Epsztejn, et al. H-Ferritin Subunit Overexpression in Erythroid Cells Reduces the Oxidative Stress Response and Induces Multidrug Resistance Properties. Blood 94 (10): 3593-3603 (1999)

Materials Safety Data Sheet

• EZ-PCR Mycoplasma Test Kit (MSDS)

Manuals and Protocols

• EZ-PCR Mycoplasma Test Kit Instructions for Use
• EZ-PCR Mycoplasma Test Kit Product Information Sheet
• Tech Tips: Mycoplasma Contamination - Effective Detection and Treatment
• Specificity of EZ-PCR Mycoplasma Test Kit

Additional Resources

• Water Bath Contamination Log Blank Worksheet (PDF)
• Incubator Contamination Log Blank Worksheet (PDF)
• Mycoplasma Facts & Figures Infographic (PDF)
• 10 Quick Tips to Prevent Mycoplasma Contamination