MSCgo Chondrogenic Differentiation Medium

Description

Product Overview

MSCgo™ Chondrogenic Differentiation Medium is a serum-free (SF) and xeno-free (XF) formulation developed for optimal differentiation of human mesenchymal stem cells (hMSC) to mature chondrocytes. The MSCgo™ Chondrogenic Differentiation Medium is validated to efficiently differeniate hMSC from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), and umbilical cord tissue (UC-MSC).

The kit includes a basal medium and supplement, containing all growth factors and supplements necessary for a complete chondrogenic differentiation medium.  No additional supplements are required.

The MSCgo™ Chondrogenic Differentiation protocol is part of a complete system for multipotency evaluation of hMSCs.  This kit enables reliable differentiation of hMSCs to mature chondrocytes without background differentiation or interruption in cellular metabolism.

Features

• Serum-free, xeno-free medium
• All required growth factors and supplements included in kit
• Reliable differentiation to mature chondrocytes
• Each lot is application tested
• Does not contain antibiotics

Components

• MSCgo Chondrogenic Differentiation Basal Medium:  100 mL
• MSCgo Chondrogenic Differentiation Supplement Mix :  10 mL

Chondrogenesis Results

Chondrogenic differentiation of hMSC in 3D spheroid culture in the formation of cartilage with a typical extracellular matrix rich of Aggrecan. Aggrecan is a proteoglycan that can be used as an indicator for cartilage formation and can be detected with Alcian Blue, a dark-blue copper-containing dye that acts as an indication of mature chondrocytes. The staining intensity can be vary using different hMSC (e.g. source, age, and passage number).

hMSC-AT

hMSC-BM

hMSC-CT

Cartilage differentiation results of hMSC from various sources after 21 day assay using MSCgo™ Chondrogenic followed by Alcian Blue staining.

hMSC-AT

hMSC-BM

hMSC-CT

Histological images of mature chondrocytes surrounded by a cartilage matrix after a 21-day differentiation assay using MSCgo™ Chondrogenic Medium followed by Toluidine blue (40x).

Specifications

Required Materials for Chondrogenic Assay

• MSCgo™ Chondrogenic Differentiation Medium (BI Cat. No. 05-220-1 and 05-221-1)
• 96-well, U-bottom, non-tissue culture treated plate (for suspension)
• MSC NutriStem® XF (BI Cat. No. 05-200-1)
• Alcian Blue 8 GX – OPTIONAL

Instructions for Use

Complete Medium Preparation

• 1.  Thaw MSCgo™ Chondrogenic Supplement Mix (05-221-1D) at room temperature.
• 2.  Dilute the Supplement Mix 1:10 in the MSCgo™ Chondrogenic Basal Medium (05-220-1B) by adding 10 mL of the Supplement Mix to 90 mL of Basal Medium.
• 3.  Store the complete medium at 2 to 8°C for up to 30 days.

Note:  The combination of the MSCgo Chondrogenic Supplement Mix into the Basal Medium generates a complete medium that is ready for use.  No additional supplements are required.

Additional information

1. J. Leber et al., Microcarrier choice and bead-to-bead transfer for human mesenchymal stem cells in serum-containing and chemically defined media. Process Biochemistry, volume 59, Part B, August 2017, Pages 255-265
2. L. Pu et al., Compared to the amniotic membrane, Wharton’s jelly may be a more suitable source of mesenchymal stem cells for cardiovascular tissue engineering and clinical regeneration. Stem Cell Research & Therapy 2017 8:72

Materials Safety Data Sheet

• MSCgo™ Chondrogenic Differentiation Medium (MSDS)
• MSCgo™ Chondrogenic Differentiation Supplement Mix (MSDS)

Manuals and Protocols

• MSCgo™ Chondrogenic Differentiation Medium Instructions for Use