Recombinant Trypsin EDTA Solution

Description

Recombinant Trypsin EDTA Solution Overview

Recombinant Trypsin EDTA Solution is a defined, animal component-free solution developed for efficient dissociation of adherent cell types from culture surfaces and tissues.  The EDTA component accelerates the dissociative action of the enzyme trypsin. This solution is suitble for sensitive cells types, including human mesenchymal stem cells (hMSC).

The Recombinant Trypsin EDTA Solution is a pure enzyme solution, which maximises the yield of functionally viable cells from culture dishes. The lack of other proteases reduces the risk of toxicity being induced in the cells. This solution does not contain undesirable proteins such as carboxypeptidase A and chymotrypsin, which are commonly found in other commercially available trypsin solutions. Recombinant Trypsin EDTA Solution is animal component-free, so eliminates the risk of viruses and other potentially detrimental and unintended agents associated with animal-derived components.

Features

• Defined, animal component-free
• Ready-to-use solution
• Accelerated enzymatic action
• Pure, recombinant trypsin – no contaminating proteases
• Compatable with cells cultured in both serum-free and serum-containing media

Specifications

Source:
Produced by submerged microbial fermentation. This solution is animal component-free, and derived from a production process that does not utilize any raw materials and/or processing aids of animal origin.

Storage & Stability:
Do not store or use past the expiration date

Quality Control:
Recombinant Trypsin Solution performance is tested on hMSC. Additional standard evaluations are pH, osmolality, and sterility tests.

Additional information

1. Hubel, A. (2018) Cryopreservation of Human Adipose Stem Cells, in Preservation of Cells: A Practical Manual, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118989883.oth4

Materials Safety Data Sheet

• Recombinant Trypsin Solution (MSDS)

Manuals and Protocols

• Recombinant Trypsin Solution Instructions for Use

Product Literature

• Technical Resources Passaging Methods for hPSCs under Feeder-Free Conditions
• BROCHURE Animal ComponentFree (ACF) Recombinant Trypsin Solutions