Vitronectin ACF (Human Recombinant), 200 µg

Description

Vitronectin

Recombinant Vitronectin ACF is a chemically-defined matrix component for the attachment of human embryonic and induced pluripotent stem cells in a feeder-free culture system. Recombinant Vitronectin ACF is a 459 amino acid, single-chain, monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. The calculated molecular weight is 52.2 kDa.

Specifications

Sterile filtered and lyophilized

• Authenticity: Verified by N-terminal and Mass Spectrometry analyses.
• Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.
• Endotoxin: Endotoxin level is < 0.1 EU/μg of protein.
• Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.
• Biological Activity: Promotes attachment of hPSC in serum-free conditions

Instructions for Use

Preparation of Vitronectin ACF, 0.5mg/ml

• Reconstitution procedure should be performed on ice.
• Spin down the vial before reconstitution.
• To the original 0.2mg vial add 0.4ml of sterile tissue culture water. DO NOT VORTEX.
• Incubate on ice for a few minutes.
• Gently mix by pipetting up and down.
• Keep on ice for immediate use (up to 1 week at 2-8°C)
• For long-term storage (3 months) aliquot and freeze at (-80)°C. Additional freeze-thaw cycles are not recommended.

Additional information

Data Sheets

• Vitronectin ACF Datasheet

Manuals and Protocols

• Vitronectin ACF coating when using NutriStem® V9 XF culture medium

Technical Resources

• Passaging Methods for hPSCs under Feeder-Free Conditions